Tell your doctor and pharmacist about all of your drugs (prescription or OTC, natural products, vitamins) and health problems. You must check to make sure that it is safe for you to take chloroquine with all of your drugs and health problems. Chloroquine proguanil malaria prophylaxis Chloroquine description Plaquenil marijuana Chemical is stable for 24 months. Once in solution, use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles. Directions for Use Chloroquine is supplied as a lyophilized powder. For a 50 mM stock, reconstitute the 150 mg in 5.82 ml sterile dH 2 O. First add 1 ml dH 2 O to the tube containing the Chloroquine diphosphate is an inhibitor of autophagy and toll-like receptors TLRs. Chloroquine diphosphate is an antimalarial and anti-inflammatory drug widely used to treat malaria and rheumatoid arthritis. - Mechanism of Action & Protocol. Chloroquine stock solutions were made at 10 mg/ml in double deionized water, and sterilized by filtration with an Acrodisc syringe filter with a 0.2 μm Tuffryn membrane Pall Life Sciences, New York. Aliquots of the sterile stock solutions were stored at -20°C until use. The appropriate amounts of stock solution were added to the media to. For all uses of chloroquine: WARNING/CAUTION: Even though it may be rare, some people may have very bad and sometimes deadly side effects when taking a drug. Do not start, stop, or change the dose of any drug without checking with your doctor. Chloroquine stock solution Addgene Lentivirus Production Protocol, Chloroquine diphosphate Autophagy Inhibitor MedChemExpress Hydroxychloroquine methotrexate sulfasalazine azathioprine lefludomideChloroquine dose for childSaposin b binds chloroquine To make 100 mM stock solution, dissolve 200 mg chloroquine in 3.9 ml water by gentle vortex. 2.ilter sterilize through a 0.22 µm filter. F 3. Store at 4. o. C Note Chloroquine solution is light sensitive. 4TLR signaling inhibition study, perform a pilot inhibitory test with the different concentrations of chloroquine ranging F Chloroquine Inhibitor - Novus Biologicals. Chloroquine treatment of ARPE-19. - PubMed Central PMC. Cholesterol stock solution for cell culture. Prepare the DNA in HBS for application to cells. 1. About 5 minutes prior to transfection, add chloroquine to each plate to 25uM chloroquine stock is 50 mM; for 3 mL media + 1 mL DNA, add 2u1. Chloroquine acts to inhibit lysosomal DNases by neutralizing vesicle pH. Chloroquine is a lysosomotropic agent that prevents endosomal acidification 1. It accumulates inside the acidic parts of the cell, including endosomes and lysosomes. This accumulation leads to inhibition of lysosomal enzymes that require an acidic pH, and prevents fusion of endosomes and lysosomes. Chloroquine is a chemotherapeutic agent for the clinical treatment of malaria. Chloroquine is able to bind to DNA, and inhibit DNA replication and RNA synthesis which in turn results in cell death. The effect of Chloroquine may also be related to the formation of a toxic heme-Chloroquine complex.